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| Registros recuperados: 992 | |
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| Muradoglu,Ferhad; Gundogdu,Muttalip; Ercisli,Sezai; Encu,Tarik; Balta,Fikri; Jaafar,Hawa ZE; Zia-Ul-Haq,Muhammad. |
| BACKGROUND: Cadmium (Cd) is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA) contents, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd. RESULTS: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased... |
| Tipo: Journal article |
Palavras-chave: Antioxidant enzymes; Cadmium; Chlorophyll; Heavy metal stress; Strawberry. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100011 |
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| Wang,Hui; Liu,Zheng; Zhang,Wenxiu; Yuan,Ziao; Yuan,Hongyi; Liu,Xueting; Yang,Chunwen; Guan,Weijun. |
| BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress... |
| Tipo: Journal article |
Palavras-chave: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042 |
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| Ontsouka,Edgar Corneille; Bertschi,Janique Sabina; Huang,Xiao; Lüthi,Michael; Müller,Stefan; Albrecht,Christiane. |
| BACKGROUND: Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMEC US) or Swiss Holstein-Friesian (bMEC CH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and α-smooth muscle actin (α-SMA.... |
| Tipo: Journal article |
Palavras-chave: Cell characterization; Flow cytometry; Gene expression; Lactation marker; Mammalian; Mammary epithelial cell. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100001 |
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| GAMBOA,JORGE; CACEDA,RICARDO; GAMBOA,ALFREDO; MONGE-C,CARLOS. |
| Red blood cell carbonic anhydrase (CA) activity has not been studied in high altitude natives. Because CA is an intraerythocytic enzyme and high altitude natives are polycythemic, it is important to know if the activity of CA per red cell volume is different from that of their sea level counterparts. Blood was collected from healthy subjects living in Lima (150m) and from twelve subjects from Cerro de Pasco (4330m), and hematocrit and carbonic anhydrase activity were measured. As expected, the high altitude natives had significantly higher hematocrits than the sea level controls (p=0.0002). No difference in the CA activity per milliliter of red cells was found between the two populations. There was no correlation between the hematocrit and CA activity. |
| Tipo: Journal article |
Palavras-chave: Carbonic anhydrase; Hypoxia; Polycythemia. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300006 |
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| Iturriaga,Rodrigo; Del Rio,Rodrigo; Idiaquez,Juan; Somers,Virend K.. |
| The carotid body (CB) is the main peripheral chemoreceptor that senses the arterial PO2, PCO2 and pH. In response to hypoxemia, hypercapnia and acidosis, carotid chemosensory discharge elicits reflex respiratory, autonomic and cardiovascular adjustments. The classical construct considers the CB as the main peripheral oxygen sensor, triggering reflex physiological responses to acute hypoxemia and facilitating the ventilatory acclimation to chronic hypoxemia at high altitude. However, a growing body of experimental evidence supports the novel concept that an abnormally enhanced CB chemosensory input to the brainstem contributes to overactivation of the sympathetic nervous system, and consequent pathology. Indeed, the CB has been implicated in several... |
| Tipo: Journal article |
Palavras-chave: Autonomic dysfunction; Carotid body; Heart failure; Metabolic syndrome; Obstructive sleep apnea; Sympathetic activation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100013 |
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| BENDER,FLORENT; MONTOYA,MARGARITA; MONARDES,VIRGINIA; LEYTON,LISETTE; QUEST,ANDREW F.G.. |
| Caveolae are small, flask-shaped invaginations of the plasma membrane present on a large number of mammalian cells. Recent results obtained with knock-out mice for the gene caveolin-1 demonstrate that expression of caveolin-1 protein is essential for caveolae formation in vivo. Caveolae are implicated in a wide variety of cellular events including transcytosis, cholesterol trafficking and as cellular centers important in coordinating signalling events. Caveolae share this role and the property of detergent insolubility with plasma membrane assemblies rich in glycosphingolipids and cholesterol, often called lipid rafts, but preferably referred to here as caveolae-like membrane domains. Due to such widespread presence and usage in cellular function, caveolae... |
| Tipo: Journal article |
Palavras-chave: Caveolae; Caveolae-like domains; Lipid rafts; Caveolin-1; Tumor suppressor; Colon carcinoma cells; Microarray analysis. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200006 |
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| Takahashi,Hiroshi; Ikeda,Kazuhiko; Ogawa,Kazuei; Saito,Syunnichi; Ngoma,Alain M; Mashimo,Yumiko; Ueda,Koki; Furukawa,Miki; Shichishima-Nakamura,Akiko; Ohkawara,Hiroshi; Nollet,Kenneth E; Ohto,Hitoshi; Takeishi,Yasuchika. |
| BACKGROUND: CD4+CD25highFOXP3+ regulatory T (Treg) cells, which include thymus-derived and peripherally induced cells, play a central role in immune regulation, and are therefore crucial to prevent graft-versus-host disease (GVHD). The increasing use of allogeneic hematopoietic stem cell transplantation (allo-HSCT) for elderly patients with thymus regression, and our case of allo-HSCT shortly after total thymectomy, raised questions about the activity of thymus-derived Treg cells and peripherally induced Treg cells, which are otherwise indistinguishable. RESULTS: We found that despite pre-transplant thymectomy or older age, both naïve and effector Treg cells, as well as naïve and effector conventional T cells, proliferated in allo-HSCT recipients. Higher... |
| Tipo: Journal article |
Palavras-chave: Regulatory T cells; Allogeneic hematopoietic stem cell transplantation; Thymus; Graft-versus-host disease. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100041 |
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| Rosas,Carlos; Sinning,Mariana; Ferreira,Arturo; Fuenzalida,Marcela; Lemus,David. |
| BACKGROUND: During the last few years it has been shown in several laboratories that Celecoxib (Cx), a non-steroidal anti-inflammatory agent (NSAID) normally used for pain and arthritis, mediates antitumor and antiangiogenic effects. However, the effects of this drug on a tumor cell line resistant to chemotherapeutical drugs used in cancer have not been described. Herein we evaluate the angiogenic and antitumor effects of Cx in the development of a drug-resistant mammary adenocarcinoma tumor (TA3-MTXR). RESULTS: Cx reduces angiogenesis in the chick embryonic chorioallantoic membrane assay (CAM), inhibits the growth and microvascular density of the murine TA3-MTXR tumor, reduces microvascular density of tumor metastases, promotes apoptosis and reduces... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Celecoxib; Tumor. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100027 |
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| Registros recuperados: 992 | |
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